Composite

Part:BBa_K3110052:Design

Designed by: Amartya Pal   Group: iGEM19_IISER_Tirupati   (2019-10-16)


lldRO1-J23117-lldRO2-Medium RBS-YebF IL12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Here we have put a lldRO1-J23117-lldRO2 along with B0032 (which is a medium strength RBS) and downstream to it, we have attached YebF-IL12 and FLAG-tag.

Source

YebF-IL12-FLAG tag was synthesized by Twist Bioscience. lldRO1-J23117-lldRO2 + B0032 and B0015 were obtained as gBlocks from IDT and these 3 parts were joined together using SOEing (Spliced Overlap Extension) PCR.

References